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    <div class="pagetitle">Viral Lipidomics</div>
    <div class="text">
        This project aims to characterize the lipid composition of the viral envelope of influenza strains of varying pathogenicity. While differences in the rate of fusion and budding of the virus from the host cell membrane have been correlated with pathogenicity, no systematic study of the contribution of differences in the composition of the viral envelope to pathogenicity has previously been attempted with modern lipidomics technology.
        <br/><br/>
        Following initial profiling using an ESI LC-MS method, nearly 150 phospholipid species from seven classes and their respective lysolipids were routinely quantitated for three strains of influenza (HKx31, PR8, and VN1203) grown in eggs.
        <br/><br/>
        The glycerophospholipid composition of the purified virions is distinct from that of mammalian cells. While phosphatidylcholine (PC) is the major component in most mammalian cell membranes, comprising typically ~50% of total glycerophosholipid, in purified virions phosphatidylethanolamine (PE) is the dominant phospholipid class. The PE:PC ratio is relatively high (5-9) in purified virions in contrast to the host material (non-infected allantoic fluid; NAF), where PE:PC is closer to 1:1.
        <br/><br/>
        <div class="subtitle">Values are expressed in pmol/ug protein<br/>
            <a href="/repositories/datasets/contents/Viral-Lipidomics/Flu_Viral_Lipidomics_Egg-grown_X31-PR8-VN1203-NAF.xlsx">Download Full Results</a>
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            NAF: Noninfected Allantoic Fluid | ND: Not detected
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